Shyam S Ganguly
Sultan Qaboos University, Oman
Title: Apolipoprotein E Gene Polymorphism with Ischemic Stroke in Omani patients: A hospital-based case–control study
Biography
Biography: Shyam S Ganguly
Abstract
Statement of the Problem: Ischemic stroke is a health concern worldwide. It has been suggested that ischemic stroke is a multifactorial disease of various etiologies involving both environmental and genetic factors. Polymorphism of apolipoprotein E (APOE) gene, which is associated with lipid metabolism, has been the focus of numerous studies as a potential risk factor for ischemic stroke. However, no such study has been carried out among Omani patients with ischemic stroke.
Objectives: The purpose of this study is to investigate the relationship between APOE gene polymorphism and lipid profiles in the risk of ischemic stroke among the Omani population.
Methodology & Theoretical Orientation: A total of 100 cases of large artery atherothrombotic and small vessel occlusion ischemic stroke were recruited from Sultan Qaboos University Hospital (SQUH). A similar number of age-matched controls were recruited from the same hospital based on some inclusion and exclusion criteria. Fasting blood samples were collected for lipid profile measurement using the standard methods and for genomic DNA extraction. APOE genotyping was performed by real-time PCR using TaqMan©SNP Genotyping Assay.